5 Simple Techniques For HPLC working
크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.The cell stage’s move charge is determined because of the merged speeds of the two pumps. By modifying the relative speeds of the two pumps, different binary mobile phases can be prepared.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
The 3 red circles are binary cell phases designed by combining equivalent volumes of the pure cellular phases. The ternary mobile phase proven through the purple circle consists of all three with the pure mobile phases.
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混合物で構成される試料を分離する。一般にステンレス製の筒の中に、微細な真球状の多孔質シリカゲルをアルキル基等で修飾した物を充填して用いる。分取目的であれば、粉砕シリカゲルも用いられる。
As it uses a loop injection, the precision of the HPLC process generally is better than a GC process. HPLC is just not restricted to risky analytes, which suggests we will assess a broader choice of compounds. Capillary GC columns, check here Then again, have extra theoretical plates, and might separate extra complicated mixtures.
Quite a few differing kinds of detectors are use to observe HPLC separations, most of which make use of the spectroscopic techniques from Chapter ten or perhaps the electrochemical strategies from Chapter 11.
Typical-period: Separates determined by polarity. Analytes with higher polarity interact much more Along with the polar stationary phase and elute afterwards.
If we switch from utilizing acetonitrile to tetrahydrofuran, for example, we see that benzoic acid elutes more promptly Which p
, a fluorescence detector presents further selectivity simply because just a few of a sample’s elements are fluorescent. Detection boundaries are as minimal as one–10 pg of injected analyte.
(HPLC) we inject the sample, that is in Alternative form, right into a liquid cell period. The mobile stage carries the sample by way of a packed or capillary column that separates the sample’s factors based on their power to partition amongst the cell section along with the stationary period. Figure twelve.
A quantitative HPLC Investigation is usually less complicated than click here a quantitative GC Assessment because a fixed volume sample loop offers a more specific and accurate injection.